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Saturday, May 16, 2020 | History

2 edition of effects of Bromodeoxyuridine on L-strain mouse cells. found in the catalog.

effects of Bromodeoxyuridine on L-strain mouse cells.

Chandrakant Manilal Pujara

effects of Bromodeoxyuridine on L-strain mouse cells.

by Chandrakant Manilal Pujara

  • 103 Want to read
  • 38 Currently reading

Published in [Toronto] .
Written in English

    Subjects:
  • Bromodeoxyuridine,
  • Cells,
  • Desoxyribonucleic acid,
  • Radiology

  • Edition Notes

    ContributionsToronto, Ont. University.
    The Physical Object
    Paginationvii, 83, [5] leaves.
    Number of Pages83
    ID Numbers
    Open LibraryOL20794415M

      5-Bromo-2′-deoxyuridine (BrdU) is frequently used as a mitotic marker in studies of cell proliferation. Recent studies have reported cytotoxic effects of BrdU on neural progenitor cells in embryonic and neonatal brains in vivo and in adult tissue studied in present study was conducted to assess whether BrdU interferes with cell proliferation and neuronal maturation in the rat Cited by: Anti-Bromodeoxyuridine shows 10% cross reaction with iodo-deoxyuridine, but no cross reaction to fluoro-deoxyuridine. No cross reaction to any endogenous thymidine or uridine. Cross reactivity with 5-Br-UTP has not been tested but it is suggested that there is a good chance for reaction, because the only difference is an absent hydroxyl group.

    Stellwagen R, Tomkins G () Differential effect of 5-bromodeoxyuridine on the concentrations of specific enzymes in hepatoma cells in culture. Proc Natl Acad Sci USA – Google Scholar Wilt F, Anderson M () The action of 5-bromodeoxyuridine on by:   1. Biochem Biophys Res Commun. Apr 28;47(2) Effects of glutamine on the two catalytic activities of glutamine synthetase in cultured mouse cells strain by:

      After 5 weeks of parabiosis the hindlimb muscles of each mouse were injured and the mice were given 5-bromodeoxyuridine (BrdU) injections 6. After muscle injury, satellite cells Cited by: Bromodeoxyuridine, also known as BrdU (MW= Da), is a synthetic analog of thymidine that is incorporated into DNA during S-phase of the cell cycle. BrdU is rapidly assimilated by proliferating cells and can be detected with anti-BrdU specific antibodies by different techniques like flow cytometry, immunofluorescence, and immunohistochemistry.


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Effects of Bromodeoxyuridine on L-strain mouse cells by Chandrakant Manilal Pujara Download PDF EPUB FB2

Distribution and virogenic effects of 5-bromodeoxyuridine in synchronized rat embryo by: Effects of bromodeoxyuridine, cytosine arabinoside and Colcemi ind upo vitron development of mouse blastocysts By MICHAEL I. SHERMAN1 AN SUD BI I ATIENZA1 From the Roche Institute of Molecular Biology, New Jersey SUMMARY Mouse blastocysts in culture have been treated with increasing concentrations of cytosine arabinoside, bromodeoxyuridin or Cited by: a specific BrdU effect and that the degree of light staining that is observed is directly related to the exposure time of a cell to BrdU The cells obtained 2 h after BrdU injection were exposed to BrdU for only the latter part of the S phase under the conditions employed.

The ligh­. Summary. The effect of BrdU on oncogenicity and growth characteristics of mouse myeloma cell lines in vitro has been studied. Oncogenicity was reduced and marked cytotoxicity was observed but globulin synthesis did not appear to be affected by less than lethal by: 1.

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS() The Effect of 5-Bromodeoxyuridine on Steroidogenesis in Mouse Adrenal Tumor Cells1 RODNEY M. WISHNOW, PATTY FEIST,2 AND MICHAEL B.

GLOWALLA3 Departments of Medicine and Microbiology, University of California, Irvine, Californiaand Veterans Administration Hospital, Long Beach, California Cited by: A partial suppression of tumorigenicity has also been demonstrated in several other mouse cell lines [10] and in an SVtransformed line of hamster cells [11].

In this paper we describe the effects of BUdR on the trans- formed phenotype in vitro and on the tumor-forming potential in vivo of a polyoma- transformed line of mouse by: 9. Mouse embryos explanted at various stages during neurulation were cultured for 20–28 h in the presence of 25– μg/ml of 5-bromodeoxyuridine (BUdR).

BUdR strongly inhibited closure of the cranial Cited by: The effect of 5-bromodeoxyuridine on cell division and differentiation of preimplantation mouse embryos By D. POLLARD,1 M. BARAN2 AND R. BACHVAROVA2 From the Department of Anatomy, Cornell University Medical College SUMMARY Mouse embryos exposed to concentrations of 5-bromodeoxyuridine (BUdR) ranging from.

BrdU not only increased the number of GFP + cells, but also speeded up the reprogramming process. GFP + cells could be observed in BrdU-treated group as early as day 7 by FACS analysis (Supplementary information, Figure S1F), and more than 30% cells were GFP + on day 14 in BrdU by: VIROL () Effect of 5-Brornodeoxyuridine and Actinomycin D on the Growth of Visna Virus in Cell Cultures HALLDOR THORMAR Institute for Experimental Pathology, University of Iceland, Keldur, Reykjavik Accepted Janu Bromodeoxyuridine (BUDR) and aetinomyein D were found to inhibit the growth of visna virus in monolayer cultures of fibroblast-like sheep by: The effects of bromodeoxyuridine (BUDr), iododeoxyuridine (IUDr), thymidine (TDr), fluorodeoxyuridine (FUDr), and fluorouracil (FU), on the growth of mouse fibroblasts (Strain L-M) and on a bromodeoxyuridine resistant subline [Stain L-M (TK −)] have been compared.

The growth of L-M cells was grossly inhibited by 25 μg/ml BUDr and 50 μg/ml IUDr while L-M (TK −) cells grew for at least 5 Cited by: Mice will be given BrdU in the drinking water ( mg/ml) daily for up to 6 weeks or - alternatively as daily i.p.

injections (~2 mg in µl of sterile saline) for up to 1 week. BrdU-containing water bottles must be shielded from light to prevent BrdU degradation and the water. BrdU promotes full-chemical induced reprogramming of MEFs.

(A) Dose-response effect of BrdU on OKSM-induced reprogramming of MEFs. Starting cell density was 4 Cited by:   5-Bromodeoxyuridine induces the differentiation of mouse neuroblastoma C to cells that morphologically resemble mature neurons. The induced differentiation can take place in the absence of DNA synthesis.

This suggests that the halogenated pyrimidine need not be incorporated into DNA to alter the phenotype of the by:   MUTATIONS at the steel (si) and dominant white spotting (W) loci in the mouse affect primordial germ cells (PGC), melanoblasts and haemopoietic stem by: Bromodeoxyuridine promotes full-chemical induction of mouse pluripotent stem cells Cell Resear ch () doi/cr; published online 7 August Our data also show that, in addition to its effect on the cell cycle, BrdU can perturb the normal differentiation of cells derived from neural stem cells.

At first glance this result seems anomalous, given the large body of work using BrdU to label, for instance, newly generated neurons in Cited by: Bromodeoxyuridine releases gene silenced by association with the histone caused by DNA methylation.

BrdU can also be used to identify microorganisms that respond to specific carbon substrates in aquatic and soil environments.

A carbon substrate added to incubations of environmental samples will cause the growth of microorganisms that can utilize that Number: Abstract.

Polytene chromosomes of Drosophila hydei have been employed to study the effect of bromodeoxyuridine (BrdU) on chromosome function and structure.

Two media have been tested for their capacity to permit substitution of thymidine by BrdU in larval salivary by: 7. Oncogenic Nras in mouse haematopoietic stem cells can increase the probability of cell division in some cells and decrease it in others; this bimodal activity explains how this single pre.

Fig. 3. Human wart virus isolated from day cultures of cells of mouse embryo skin; stained with PTA.

tures inoculated with saline or boiled virus. In mouse cell experiments, the initial cytotoxicity wasless severe thanthatob- served in experiments with human skin cultures. The mouse cells were fully re- coveredfromthe initial cytotoxicity 4or 5 days after inoculation.

At this time,Cited by: Bromodeoxyuridine (BrdU) is one such common nucleotide analog that is classically used to track label‐retaining cells (LRCs) after a prolonged “washout” period that dilutes the label within the more rapidly cycling transient‐amplifying (TA) cells.Title:Effect of Nicotine on the Proliferation and Differentiation of Mouse Induced Pluripotent Stem Cells VOLUME: 19 ISSUE: 30 Author(s):T.

Ishizuka, H. Goshima, A. Ozawa and Y. Watanabe Affiliation:Department of Pharmacology, National Defense Medical College,Namiki, Tokorozawa, SaitamaJapan. Keywords:Nicotine, nicotinic acetylcholine receptor, DNA synthesis.